Exosomes are extracellular vesicles, or small bubbles, released from cells that act as shuttles for genetic information, proteins and messenger RNA to other cells. This usually happens in response to injuries. Generally speaking, Exosomes carry healthy and lost information and insert this to target cells. Exosomes released by young stem cells have shown to be very powerful in regulating regenerative processes in the body and assisting in rejuvenation.

Exosomes from stem cells contain valuable cell information consisting of proteins and genetic information the cell needs to function correctly. These stem cell derived Exosomes have also shown to assist in transferring NK cells (Killer cells) into T-Regulator cells (Helper cells). By that they assist in calming an over-reactive immune system and modulating it to respond in a coordinated fashion. They can also be used as a delivery system for medication.

Exosomes are administered as an IV treatment or IV push. As a part of joint rejuvenation therapy, exosomes are administered directly into the affected joint area. The normal concentration is 1 billion exosomes per 1ml. One treatment may consist of up to 15 billion exosomes. The dosing is individual to every patient and there is no set or established protocol. In some cases, exosomes may be combined with focused ultrasound, frequency therapy or regional hyperthermia which may help attract them to a certain area in the body.

Exosome therapy is usually performed on an outpatient basis. Most patients should expect to leave the clinic without any down time. The patient will not experience any discomfort. Less than 10% of patients have reported developing a mild fever, headache, nausea or vomiting. However, these side effects have never lasted more than three days and usually resolve within 24 hours. No long-term negative side effects have been reported.

In order to gain an optimal response from exosome therapy, we recommend the following:

• Refraining from anti-inflammatory medication
• No over strenuous activity for the first 24–48 hours
• Resume cardio workouts after 3–4 weeks
• After four weeks resume any weight lifting and running activity if part of your prior routine

Both stem cells and exosomes have their place in a treatment protocol. This much depend on what the treating physician is trying to accomplish. When performed correctly, treatment with exosomes and/or stem cell therapy is safe. Therapy with exosomes may carry a lower risk of complications as exosomes do not require an invasive surgical procedure for harvesting.
Also, the use of embryonic stem cells may be connected to the risk of developing tumors. Stem cell derived exosomes can be safely harvested and do not proliferate but rather transfer valuable biological signals to the recipient’s tissues and facilitate the normalization of various pathological processes.

There are different types of exosomes. There is a general standard exosome solution produced from placental derived mesenchymal stem cells. These stem cells are GMP laboratory stem cells, which have been screened and certified. The end product contains many anti-inflammatory cytokines and immune modulatory proteins.
It is also possible to produce different kinds of exosomes. Some involve the use of a patient’s own tissue to provide specific markers in combination with laboratory produced exosomes. There are several techniques that involve frequency medicine, focused ultrasound or targeted heat to attract the exosomes to a certain area of the body.
The legalities in each country also play a role in what is possible. The treating doctor can decide if individualization is necessary and what that best type of individualization may be.

Exosomes are sourced from laboratory grade, GMP certified mesenchymal stem cells. Placental mesenchymal stem cells produce the exosomes with the correct expression for our use. We have our own GMP labs in Germany and Shanghai in which we can produce exosomes. We also use some exosomes from  third-party laboratories, which are FDA certified and GMP compliant laboratories, as a basic product.

Exosomes are suspended in saline and must be kept frozen.

The starting material (mesenchymal stem cells) are GMP laboratory grade, meaning they have been screened for disease, viruses and bacteria. Once the exosomes have been extracted, the stem cells are discarded. The finished exosomes solution is sterile filtered and tested according to the standard pharmaceutical regulations for sterility.

This would be extremely unlikely. It is possible though to have an immune response to viruses due to the immune modulation. This is not viral reactivation.

This is extremely unlikely as there are no immune markers on the surface of the exosomes. They are not cells or tissue. It may be possible to experience die-off reaction after receiving exosomes as these activate the natural killer cells to become T-Regulator cells. For this reason, we always give a small test dose before applying a larger dose.

No, as they are NOT embryonic stem cells. Exosomes do not multiply. Exosomes transfer valuable biological signals to the recipient’s tissues and facilitate the normalization of various pathological processes.

In some cases a follow-up treatment may be advised. There is no sustainable data about standard dosing and the treatment is as individual as the response. Some patients may need a single application, while others need multiple doses or a small booster therapy. In some cases, therapy may start with a small initial dose which is increased over time.

Exosomes insert information in the cell. The information is basically ‘This is what a healthy cell is like and this is how to get there”. This process happens in 2 stages. In the initial phase the exosome inserts proteins into the cell. We know exosomes contain a whole array of inflammation regulating proteins. These basically quench the immediate inflammatory response. The exosomes also insert messenger RNA into the cell which is the recipe and instructions for the cell to make its own inflammation regulating substances. One has to keep in mind that MCAS is only possible with dysfunctional or damaged mast cells. Exosomes are thought to insert the missing information into the mast cells to help normalize or stabilize them and prevent them from being dysfunctional.

Exosomes trigger a so-called bi-phasic response. First, an immediate reaction. This usually last about 24 hours until the initial proteins have been broken down. Then the messenger RNA, which has been inserted into the target cells becomes active and helps ‘reprogram’ the cell. This usually takes 6-8 weeks. So the timeline is about 8-10 weeks. The continued effects may continue for months afterwards.

The bi-phasic response can be accompanied with a certain set of symptoms. These may indicate that the exosomes are assisting in the repair and regulation process. In the initial phase patients generally report a reduction of their inflammatory symptoms. The following weeks can be accompanied with light flu-like symptoms and fluctuating energy. After 6-8 weeks the second phase of the response takes place in which the information the exosomes transferred to the cells is used by the cells to regulate and reprogram the cell function and aid in repair processes. This process can again result in episodes with low energy or light flew like symptoms and a flare up of your individual symptoms. Typically, early benefits of therapy are observed in 3-4 months. However, it is not uncommon, to see the benefits after 9 to 12 months.

Immune modulation is part of EVO’s Five Steps to Health approach which uses supportive therapies to reestablish foundational body systems and functions. This includes establishing a healthy immune response by balancing the immune cells and sensitizing the immune system to pathogens. An immune response may be enhanced or suppressed by exosomes depending on their cell of origin and its functional state. Stem cell derived exosomes have been reported to impair dendritic cell maturation and to regulate the activation, differentiation, and proliferation of B cells. They have been shown to control natural killer cell activity and to suppress the innate immune response (IIR). Studies reporting the role of exosomes on T lymphocyte modulation are controversial. Therefore, while exosomes may assist in immune modulation in some cases, we would still consider at other immune modulation therapies like thymus therapy to support the immune function.

Cancer Exosomes can cause cancer to grow. These are not to be confused with exosomes
derived from stem cells which we use in treatment. Stem cell derived exosomes can actually
be indicated in the treatment of some cancers as they can insert the missing proteins into the cancer cells and stop their replication.

Research has shown that exosomes can penetrate the blood-brain barrier and stimulate neuronal differentiation, neuronal growth, and suppress inflammatory processes within the brain tissue.

Recent research has shown that stem cell-derived exosomes have an anti-inflammatory potential and induce high levels of anti- inflammatory cytokines and can therefore assist to regulate the inflammatory response. In addition, they can also inhibit abnormal macrophage activation. The macrophage is a large white blood cell that is an integral part of our immune system. Its job is to locate microscopic foreign bodies and ‘eat’ them. However, disproportional macrophage activation can induce undesirable inflammatory processes.

Chronic infections have a lot in common with autoimmune diseases, including dysregulated immune response incapable of protecting our body from intruding pathogens (bacteria, viruses, molds etc.) while attacking its own tissues and organs thus causing significant structural damage. One of the key mechanisms controlling the direction of immune responses is a balance between specific immune cells involved in protection vs autoimmune responses, the Th17/Treg ratio. Exosomes from mesenchymal stem cells have shown to normalize the Th17/Treg ratio and bring the deviant immune response back to normal.